|
KMID : 0603820130190030213
|
|
Journal of Experimental & Biomedical Science
2013 Volume.19 No. 3 p.213 ~ p.223
|
|
|
Molecular Epidemiology of Cryptococcus neoformans/Cryptococcus gattii Complex Isolates from Pigeon Droppings in Korea
|
|
Chang Kyung-Soo
|
|
|
Abstract
|
|
|
|
The objectives of this study are to develop a molecular diagnosis to differentiate serotypes and mating types of C. neoformans/C. gattii complex isolates from pigeon droppings in Korea and to elucidate molecular epidemiology of the isolates. Phenotypes and genotypes of C. neoformans/C. gattii complex isolates were identified by biochemical properties and PCR using specific CNLAC1 gene, respectively. To classify serotypes and mating?types of C. neoformans/C. gattii complex isolates, the five reference strains and thirty three isolates in Korea were investigated by restriction fragment length polymorphism (RFLP) analysis using CNLAC1 gene for varieties, by random amplified polymorphic DNA (RAPD) for serotyping, and by PCR using specific primer sets for mating typing. All isolates in Korea were belonged to C. neoformans var. grubii (serotype A) by RFLP and RAPD patterns which showed high sensitivity and specificity. Therefore, RFLP and RFLP were available to differentiate varieties and serotypes of C. neoformans. Amplification patterns of the five reference strains by specific PCR for mating typing were differentiable, and all isolates were classified into MATα. All C. neoformans environmental isolates in Korea were Cr. neoformans serotype A and MATα which is a more virulent pathogen. This study suggests that RFLP and RAPD are rapid and correct molecular diagnosis tools for epidemiology of C. neoformans/C. gattii complex isolates.
|
|
|
KEYWORD
|
|
|
C. neoformans/C. gattii complex, Pigeon droppings, Molecular epidemiology, RFLP, RAPD
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|